Advantages and Disadvantages of Brightfield Microscopy

Advantages of brightfield microscopy
It works with minimal adjustments when viewing images.
It can be used to view stained and unstained images.
The optics of the microscope do not change the color of the sample.
Microscopes can be adjusted and modified to allow for better viewing, such as mounting a camera to create a digital microscope, or image illumination through methods such as using fluorescent dyes on the sample and observing in the dark, creating a darkfield microscope.
Disadvantages of brightfield microscopy
The aperture diaphragm may produce a large contrast, which may distort the output of the image, therefore, it is best to use the iris diaphragm.
It cannot be used to view living specimens, such as bacterial cells. Only fixed specimens can be seen under a brightfield microscope.
The maximum magnification of a brightfield microscope is 100x, but modifications can readjust the magnification to 1000x, which is the optimal magnification for bacterial cells.
It has very low contrast, so most samples must be stained to be visualized.
Using oil immersion may distort the image
Using coverslips may damage the sample
Staining may introduce unwanted detail into the sample or contaminate the sample.
Staining the sample before viewing it under a brightfield microscope is cumbersome.
Microscopes require powerful light sources for magnification, and sometimes the light sources can generate large amounts of heat that can damage or kill the specimen.